Tohoku Fukushi University

11年以前の主な研究業績等  【5点まで】

基本情報

氏名 土谷 昌広
氏名(カナ) ツチヤ マサヒロ
氏名(英語) Tsuchiya Masahiro

種類

学術論文

著書、学術論文等の名称

Synthesis and intracellular transportation of type I procollagen during functional differentiation of odontoblasts.

単著・共著の別

共著

発行又は発表の年月

2009/05

発行所、発行雑誌等又は発表学会等の名称

Histochem Cell Biol. 131(5)

概要

The expression of type I collagen, the most component of dentin extracellular matrix proteins (ECMs) in odontoblast is correlated with the activity of dentin formation. Since odontoblast possesses a distinct cellular process for protein transport into the dentinal tubule, it is important to examine the intracellular protein localization. However, a study focusing on odontoblast processes has not been performed. Type I collagen is synthesized as procollagen, which is immediately converted to collagen upon secretion. After characterization of antiserum to rat type I procollagen, we investigated the intracellular localization of type I procollagen in odontoblasts during and after dentinogenesis, using immunohistochemistry and in situ hybridization. The level of mRNA expression decreased during dentinogenesis, whereas the intracellular localization of type I procollagen in odontoblast processes become more distinct. The percentage of dentinal tubules with type I procollagen increased significantly with aging. Odontoblasts in pulp horn, in particular, showed moderate expression of type I procollagen after dentinogenesis. Since loss of occlusion also caused a significant decrease in type I procollagen, we concluded that occlusal stimulation activated type I procollagen synthesis in odontoblasts. We also suggest that analysis of intracellular transport of type I procollagen via odontoblast processes may be a new approach to evaluation of odontoblast function.

ページ数等

583-91.

【共・共著の場合のみ】共・共著者名

Sato S, Tsuchiya M, Komaki K, Kusunoki S, Tsuchiya S, Haruyama N, Takahashi I, Sasano Y, Watanabe M.